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Ressonância Paramagnética Eletrônica×Calorimetria de Titulação Isotérmica×
ÁreaEspectroscopiaEspectroscopia
FamíliaProcess / pipelineProcess / pipeline
Ano de origem19451989
Autor originalEvgeny ZavoiskiiTerrence Wiseman
TipoSpectroscopic techniqueBiophysical technique
Fonte seminalZavoiskii, E. K. (1945). Paramagnetic relaxation of liquid solutions for perpendicular fields. Zhurnal Eksperimental'noi i Teoreticheskoi Fiziki, 15(6), 378-380. link ↗Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. DOI ↗
Outros nomesEPR spectroscopy, ESR, electron spin resonanceITC, isothermal calorimetry, microcalorimetry
Relacionados33
ResumoElectron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a spectroscopic technique that detects and characterizes unpaired electrons in molecules and materials. Discovered by Zavoiskii in 1945, EPR measures the absorption of microwave radiation by paramagnetic species in a magnetic field, providing information about electron spin states, local electronic environment, and molecular dynamics.Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization.
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ScholarGateComparar métodos: Electron Paramagnetic Resonance · Isothermal Titration Calorimetry. Recuperado em 2026-06-19 de https://scholargate.app/pt/compare