Comparar métodos
Examine os métodos selecionados lado a lado; as linhas que diferem ficam destacadas.
| Montagem de Transcriptoma De Novo× | Análise de Triagem CRISPR× | |
|---|---|---|
| Área | Bioinformática | Bioinformática |
| Família | Process / pipeline | Process / pipeline |
| Ano de origem≠ | 2011 | 2013 |
| Autor original≠ | Aviv Regev | Feng Zhang |
| Tipo≠ | Sequence assembly pipeline | High-throughput genetic screen pipeline |
| Fonte seminal≠ | Grabherr, M. G., Haas, B. J., Yassour, M., Levin, J. Z., Thompson, D. A., Amit, I., ... & Regev, A. (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 29(7), 644-652. DOI ↗ | Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87. DOI ↗ |
| Outros nomes≠ | transcriptome assembly, de novo assembly, RNA-Seq assembly | CRISPR pooled screen, genetic screen analysis |
| Relacionados | 3 | 3 |
| Resumo≠ | De novo transcriptome assembly reconstructs full-length messenger RNA sequences directly from sequencing reads without requiring a reference genome. Pioneered by Regev, Haas, and colleagues, this pipeline enables transcript discovery in non-model organisms and detection of novel isoforms, fusion genes, and splice variants. | CRISPR screen analysis processes data from pooled genetic screens using CRISPR-Cas9 to identify genes required for cell growth, survival, or phenotype in specific conditions. Developed by Zhang, Sanjana, and others, this computational pipeline transforms sequencing readouts of guide RNA abundances into ranked lists of functional genes. |
| ScholarGateConjunto de dados ↗ |
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