Comparar métodos
Examine os métodos selecionados lado a lado; as linhas que diferem ficam destacadas.
| Análise de Triagem CRISPR× | Montagem de Transcriptoma De Novo× | |
|---|---|---|
| Área | Bioinformática | Bioinformática |
| Família | Process / pipeline | Process / pipeline |
| Ano de origem≠ | 2013 | 2011 |
| Autor original≠ | Feng Zhang | Aviv Regev |
| Tipo≠ | High-throughput genetic screen pipeline | Sequence assembly pipeline |
| Fonte seminal≠ | Shalem, O., Sanjana, N. E., Hartenian, E., Shi, X., Scott, D. A., Mikkelsen, T. S., ... & Zhang, F. (2014). Genome-scale CRISPR-Cas9 knockout screening in human cells. Science, 343(6166), 84-87. DOI ↗ | Grabherr, M. G., Haas, B. J., Yassour, M., Levin, J. Z., Thompson, D. A., Amit, I., ... & Regev, A. (2011). Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nature Biotechnology, 29(7), 644-652. DOI ↗ |
| Outros nomes≠ | CRISPR pooled screen, genetic screen analysis | transcriptome assembly, de novo assembly, RNA-Seq assembly |
| Relacionados | 3 | 3 |
| Resumo≠ | CRISPR screen analysis processes data from pooled genetic screens using CRISPR-Cas9 to identify genes required for cell growth, survival, or phenotype in specific conditions. Developed by Zhang, Sanjana, and others, this computational pipeline transforms sequencing readouts of guide RNA abundances into ranked lists of functional genes. | De novo transcriptome assembly reconstructs full-length messenger RNA sequences directly from sequencing reads without requiring a reference genome. Pioneered by Regev, Haas, and colleagues, this pipeline enables transcript discovery in non-model organisms and detection of novel isoforms, fusion genes, and splice variants. |
| ScholarGateConjunto de dados ↗ |
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