Sammenlign metoder
Gjennomgå de valgte metodene side om side; rader som avviker, er uthevet.
| Transwell Assay× | Scratch Wound Assay× | |
|---|---|---|
| Fagfelt | Biomaterialer | Biomaterialer |
| Familie | Process / pipeline | Process / pipeline |
| Opprinnelsesår≠ | 1962 | 2007 |
| Opphavsperson≠ | Stephen Boyden | Liang, Park, and Guan |
| Type≠ | Migration/invasion assay | Migration assessment |
| Opprinnelig kilde≠ | Boyden, S. (1962). The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. Journal of Experimental Medicine, 115(3), 453-466. DOI ↗ | Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. DOI ↗ |
| Alias | Boyden chamber assay, chemotaxis assay, invasion chamber assay | wound healing assay, gap closure assay, migration assay |
| Relaterte | 4 | 4 |
| Sammendrag≠ | The Transwell assay (also called the Boyden chamber assay after its originator Stephen Boyden) is a quantitative method for measuring cell migration and invasion in response to chemical gradients or through matrix barriers. The assay uses a membrane insert with defined pore size suspended in a multi-well plate: cells are placed in the upper chamber, a chemoattractant is placed in the lower chamber, and cells that successfully migrate through the pores accumulate in the lower chamber, where they can be counted or visualized. Variants that coat the insert with matrix proteins (Matrigel, collagen) enable measurement of invasion capacity. The Transwell assay is a gold-standard method in cell biology for evaluating cell motility, tumor metastatic potential, and the effects of growth factors and inhibitory compounds. | The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential. |
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