Methoden vergelijken

	Analyse van metabolomics op enkele cellen	Metabolomicsanalyse
Vakgebied	Bio-informatica	Bio-informatica
Familie	Process / pipeline	Process / pipeline
Jaar van ontstaan	2013–2021 (emerging field; major methods established ~2019–2021)	1998–2002
Grondlegger	Multiple groups; key early platforms: Alexandrov lab (SpaceM), Bhatt/Bhattacharya groups	Oliver et al. (coining of 'metabolomics'); Oliver Fiehn (systematic framework)
Type	Analytical pipeline	Quantitative omics pipeline
Oorspronkelijke bron	Rappez, L., Stadler, M., Triana, S., Gathungu, R. M., Ovchinnikova, K., Phapale, P., Heikenwalder, M., & Alexandrov, T. (2021). SpaceM reveals metabolic states of single cells. Nature Methods, 18(7), 799–805.	Fiehn, O. (2002). Metabolomics — the link between genotypes and phenotypes. Plant Molecular Biology, 48(1-2), 155–171.
Aliassen	scMetabolomics, single-cell metabolic profiling, single-cell mass spectrometry metabolomics, SC-MS metabolomics	metabolome profiling, metabolic profiling, metabonomics, metabolite profiling
Verwant	4	6
Samenvatting	Single-cell metabolomics analysis measures the small-molecule metabolite content of individual cells, revealing cell-to-cell metabolic heterogeneity that bulk methods obscure by averaging. Rooted in mass spectrometry and microfluidics advances, it enables researchers to map metabolic states across cell populations, identify rare subpopulations, and link metabolic phenotypes to cellular function — providing a functional complement to transcriptomics and proteomics at single-cell resolution.	Metabolomics analysis is the large-scale, systematic measurement of small-molecule metabolites in a biological sample to characterise the metabolome — the complete set of metabolic intermediates and products present under defined conditions. By coupling high-throughput analytical platforms such as mass spectrometry (MS) or nuclear magnetic resonance (NMR) spectroscopy with multivariate statistics and pathway databases, metabolomics bridges the genotype–phenotype gap and captures the downstream functional output of genes, transcripts, and proteins in real time.