Methoden vergelijken

	Michaelis-Menten Kinetiek	Patch-Clamp Elektrofyziologie	Schild-analyse
Vakgebied	Farmacologie	Farmacologie	Farmacologie
Familie	Process / pipeline	Process / pipeline	Process / pipeline
Jaar van ontstaan	1913	1976	1947
Grondlegger	Leonor Michaelis and Maud Menten	Erwin Neher and Bert Sakmann	Henry Schild
Type	mechanistic model	ion channel screening	antagonism quantification
Oorspronkelijke bron	Michaelis, L., & Menten, M. L. (1913). Die Kinetik der Invertinwirkung. Biochemische Zeitschrift, 49, 333-369.	Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802.	Schild, H. O. (1947). pA, a new scale for the measurement of drug antagonism. Journal of Physiology, 106(3), 337-357.
Aliassen	MM kinetics, Michaelis constant, Vmax	patch clamp, whole-cell recording, ion channel assay	Schild plot, pA2
Verwant	2	3	3
Samenvatting	Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. Developed by Leonor Michaelis and Maud Menten in 1913, this foundational framework models enzyme catalysis through the rapid-equilibrium approximation and enables prediction of drug metabolism rates in pharmacokinetics.	Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.	Schild analysis is a quantitative method for characterizing competitive receptor antagonism developed by Henry Schild in 1947. It uses dose-response curves in the presence and absence of antagonist to estimate the antagonist affinity constant (pA2), enabling standardized comparison of antagonist potency across drugs and experimental systems.