Salīdzināt metodes
Apskatiet izvēlētās metodes blakus; rindas, kas atšķiras, ir izceltas.
| MALDI-TOF× | Cirkulārais dihroisms× | |
|---|---|---|
| Nozare | Spektroskopija | Spektroskopija |
| Saime | Process / pipeline | Process / pipeline |
| Izcelsmes gads≠ | 1988 | 1969 |
| Autors≠ | Michael Karas | Jean-Claude Fasman |
| Tips≠ | Ionization and mass analysis technique | Spectroscopic method |
| Pirmavots≠ | Karas, M., & Hillenkamp, F. (1988). Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Analytical Chemistry, 60(20), 2299-2301. DOI ↗ | Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116. DOI ↗ |
| Citi nosaukumi | MALDI mass spectrometry, MALDI-TOF-MS, laser desorption mass spectrometry | CD spectroscopy, circular dichroism, CD analysis |
| Saistītās | 3 | 3 |
| Kopsavilkums≠ | Matrix-Assisted Laser Desorption/Ionization (MALDI) combined with Time-of-Flight (TOF) mass analysis, or MALDI-TOF, is a soft ionization mass spectrometry technique that gently ionizes intact biomolecules and volatile organic compounds, then measures their mass-to-charge ratio by measuring flight time through a field-free drift region. Introduced independently by Karas, Hillenkamp, and Tanaka in 1988, MALDI-TOF revolutionized proteomics, microbiology, and organic analysis by enabling mass determination of proteins and polymers exceeding 100 kDa. | Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding. |
| ScholarGateDatu kopa ↗ |
|
|