手法を比較

	スクラッチアッセイ	生細胞/死細胞アッセイ
分野	生体材料学	生体材料学
系統	Process / pipeline	Process / pipeline
提唱年	2007	2000
提唱者	Liang, Park, and Guan	Invitrogen/Molecular Probes
種類	Migration assessment	Dual-dye viability assay
原典	Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333.	Molecular Probes (2004). LIVE/DEAD Viability/Cytotoxicity Kit user guide. Invitrogen Corporation.
別名	wound healing assay, gap closure assay, migration assay	calcein-AM/propidium iodide, SYTO/PI staining, fluorescent viability stain
関連	4	4
概要	The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential.	The Live/Dead assay is a fluorescence-based method for simultaneously identifying live and dead cells using two complementary dyes. The assay combines calcein-AM (or SYTO fluorophores), which generates bright green fluorescence in living cells with intact esterase activity, with propidium iodide (PI), which produces red fluorescence in dead cells with compromised membrane integrity. Commercially developed by Molecular Probes and now part of Thermo Fisher's portfolio, the Live/Dead kit is widely used to evaluate cell viability on biomaterial scaffolds, in tissue constructs, and following drug or toxin exposure.