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| ミカエリス・メンテン速度論× | パッチクランプ電気生理学× | |
|---|---|---|
| 分野 | 薬理学 | 薬理学 |
| 系統 | Process / pipeline | Process / pipeline |
| 提唱年≠ | 1913 | 1976 |
| 提唱者≠ | Leonor Michaelis and Maud Menten | Erwin Neher and Bert Sakmann |
| 種類≠ | mechanistic model | ion channel screening |
| 原典≠ | Michaelis, L., & Menten, M. L. (1913). Die Kinetik der Invertinwirkung. Biochemische Zeitschrift, 49, 333-369. link ↗ | Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗ |
| 別名 | MM kinetics, Michaelis constant, Vmax | patch clamp, whole-cell recording, ion channel assay |
| 関連≠ | 2 | 3 |
| 概要≠ | Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. Developed by Leonor Michaelis and Maud Menten in 1913, this foundational framework models enzyme catalysis through the rapid-equilibrium approximation and enables prediction of drug metabolism rates in pharmacokinetics. | Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment. |
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