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| フローサイトメトリー解析× | Caco-2細胞透過性アッセイ× | |
|---|---|---|
| 分野 | 薬理学 | 薬理学 |
| 系統 | Process / pipeline | Process / pipeline |
| 提唱年≠ | 1976 | 1989 |
| 提唱者≠ | Leonard Herzenberg | Ingrid Hidalgo |
| 種類≠ | cell analysis and sorting | absorption screening |
| 原典≠ | Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827. DOI ↗ | Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749. DOI ↗ |
| 別名 | FACS, fluorescence-activated cell sorting, cell analysis | Caco-2 assay, intestinal permeability, ADME screening |
| 関連 | 3 | 3 |
| 概要≠ | Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology. | The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport. |
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