手法を比較
選択した手法を並べて確認できます。異なる行はハイライト表示されます。
| 電子常磁性共鳴× | 等温滴定熱量測定× | |
|---|---|---|
| 分野 | 分光学 | 分光学 |
| 系統 | Process / pipeline | Process / pipeline |
| 提唱年≠ | 1945 | 1989 |
| 提唱者≠ | Evgeny Zavoiskii | Terrence Wiseman |
| 種類≠ | Spectroscopic technique | Biophysical technique |
| 原典≠ | Zavoiskii, E. K. (1945). Paramagnetic relaxation of liquid solutions for perpendicular fields. Zhurnal Eksperimental'noi i Teoreticheskoi Fiziki, 15(6), 378-380. link ↗ | Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. DOI ↗ |
| 別名 | EPR spectroscopy, ESR, electron spin resonance | ITC, isothermal calorimetry, microcalorimetry |
| 関連 | 3 | 3 |
| 概要≠ | Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a spectroscopic technique that detects and characterizes unpaired electrons in molecules and materials. Discovered by Zavoiskii in 1945, EPR measures the absorption of microwave radiation by paramagnetic species in a magnetic field, providing information about electron spin states, local electronic environment, and molecular dynamics. | Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization. |
| ScholarGateデータセット ↗ |
|
|