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ATR-FTIR×円偏光二色性×
分野分光学分光学
系統Process / pipelineProcess / pipeline
提唱年19611969
提唱者Joop FahrenfortJean-Claude Fasman
種類Vibrational spectroscopy techniqueSpectroscopic method
原典Harrick, N. J. (1960). Study of physics of internal reflection from metals. Journal of Physics and Chemistry of Solids, 13(2), 143-155. link ↗Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116. DOI ↗
別名ATR-IR, attenuated total reflectance, FTIR spectroscopyCD spectroscopy, circular dichroism, CD analysis
関連33
概要Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) spectroscopy is a variant of conventional FTIR that measures infrared absorption through evanescent-wave interrogation of samples in direct contact with a high-refractive-index crystal. Developed by Harrick and Fahrenfort in the 1960s, ATR-FTIR is now the dominant form of FTIR spectroscopy, enabling rapid, non-destructive characterization of organic compounds, polymers, coatings, and biological materials without extensive sample preparation.Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding.
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ScholarGate手法を比較: ATR-FTIR · Circular Dichroism. 2026-06-18に以下より取得 https://scholargate.app/ja/compare