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| Elektroszálazás× | MTT/MTS vizsgálat× | |
|---|---|---|
| Tudományterület | Bioanyagok | Bioanyagok |
| Módszercsalád | Process / pipeline | Process / pipeline |
| Keletkezés éve≠ | 1934 | 1983 |
| Megalkotó≠ | Anton Formhals | Tatsuro Mosmann |
| Típus≠ | Fiber fabrication process | Colorimetric assay |
| Alapmű≠ | Formhals, A. (1934). Process and apparatus for preparing artificial threads. U.S. Patent 1,975,504. link ↗ | Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. DOI ↗ |
| Alternatív nevek≠ | electrospun fiber production, electrostatic fiber spinning | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay |
| Kapcsolódó≠ | 3 | 4 |
| Összefoglaló≠ | Electrospinning is an electrostatic fiber fabrication process that uses a high electric field to draw polymer solutions or melts into nanoscale fibers. Developed by Anton Formhals in the 1930s and refined by researchers including Darrell Reneker in the 1990s, the technique has become foundational to biomaterials engineering, enabling the creation of porous scaffolds for tissue engineering and drug delivery systems. | The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development. |
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