השוואת שיטות

	ניתוח פרוטאומי	יישור רצפים
תחום	ביואינפורמטיקה	ביואינפורמטיקה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	1994–2003 (term coined 1994; shotgun proteomics established early 2000s)	1970 (global alignment); 1981 (local alignment)
הוגה השיטה	Marc Wilkins, Matthias Mann, Ruedi Aebersold (proteome/mass spectrometry foundations)	Saul B. Needleman & Christian D. Wunsch (global); Temple F. Smith & Michael S. Waterman (local)
סוג	Quantitative omics pipeline	Computational sequence analysis technique
מקור מכונן	Wilkins, M. R., Sanchez, J.-C., Gooley, A. A., Appel, R. D., Humphery-Smith, I., Hochstrasser, D. F., & Williams, K. L. (1996). Progress with proteome projects: Why all proteins expressed by a genome should be identified and how to do it. Biotechnology and Genetic Engineering Reviews, 13(1), 19–50.	Needleman, S. B., & Wunsch, C. D. (1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology, 48(3), 443–453.
כינויים	proteomics, mass spectrometry-based proteomics, shotgun proteomics, quantitative proteomics	pairwise alignment, multiple sequence alignment, MSA, sequence comparison
קשורות	6	6
תקציר	Proteomics analysis is a systematic pipeline for identifying and quantifying proteins in biological samples using mass spectrometry. Starting from raw spectral data, the workflow searches protein sequence databases, estimates abundance across conditions, applies statistical tests for differential expression, and maps findings onto biological pathways. It complements transcriptomics by capturing post-translational regulation and actual protein abundance, and is central to biomarker discovery, drug-target identification, and systems biology.	Sequence alignment is a foundational bioinformatics technique that arranges two or more DNA, RNA, or protein sequences to reveal regions of similarity, infer evolutionary relationships, identify functional domains, and map sequencing reads to reference genomes. It underpins virtually every downstream genomic analysis, from variant calling and gene expression quantification to phylogenetics and structural annotation.