השוואת שיטות

	קינטיקת מייכליס-מנטן	אלקטרופיזיולוגיה בשיטת Patch-Clamp
תחום	פרמקולוגיה	פרמקולוגיה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	1913	1976
הוגה השיטה	Leonor Michaelis and Maud Menten	Erwin Neher and Bert Sakmann
סוג	mechanistic model	ion channel screening
מקור מכונן	Michaelis, L., & Menten, M. L. (1913). Die Kinetik der Invertinwirkung. Biochemische Zeitschrift, 49, 333-369.	Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802.
כינויים	MM kinetics, Michaelis constant, Vmax	patch clamp, whole-cell recording, ion channel assay
קשורות	2	3
תקציר	Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. Developed by Leonor Michaelis and Maud Menten in 1913, this foundational framework models enzyme catalysis through the rapid-equilibrium approximation and enables prediction of drug metabolism rates in pharmacokinetics.	Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.