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| Électrophysiologie par patch-clamp× | Cinétique de Michaelis-Menten× | |
|---|---|---|
| Domaine | Pharmacologie | Pharmacologie |
| Famille | Process / pipeline | Process / pipeline |
| Année d'origine≠ | 1976 | 1913 |
| Auteur d'origine≠ | Erwin Neher and Bert Sakmann | Leonor Michaelis and Maud Menten |
| Type≠ | ion channel screening | mechanistic model |
| Source fondatrice≠ | Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802. DOI ↗ | Michaelis, L., & Menten, M. L. (1913). Die Kinetik der Invertinwirkung. Biochemische Zeitschrift, 49, 333-369. link ↗ |
| Alias | patch clamp, whole-cell recording, ion channel assay | MM kinetics, Michaelis constant, Vmax |
| Apparentées≠ | 3 | 2 |
| Résumé≠ | Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment. | Michaelis-Menten kinetics describes the rate of enzyme-catalyzed reactions as a function of substrate concentration. Developed by Leonor Michaelis and Maud Menten in 1913, this foundational framework models enzyme catalysis through the rapid-equilibrium approximation and enables prediction of drug metabolism rates in pharmacokinetics. |
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