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	Spéciation des métaux lourds	Essais écotoxicologiques
Domaine	Génie de l'environnement	Génie de l'environnement
Famille	Process / pipeline	Process / pipeline
Année d'origine	1979	1975
Auteur d'origine	Tessier and hydrogeochemists	EPA and OECD
Type	analytical and geochemical modeling pipeline	experimental measurement and analysis pipeline
Source fondatrice	Tessier, A., Campbell, P. G. C., & Bisson, M. (1979). Sequential Extraction Procedure for the Speciation of Particulate Trace Metals. Analytical Chemistry, 51(7), 844–851.	OECD. (2011). Test Guidelines for Chemicals. OECD Publishing.
Alias	metal speciation, metal partitioning, bioavailability assessment, speciation analysis	toxicity testing, aquatic bioassay, ecotoxicity assessment, organism exposure testing
Apparentées	3	3
Résumé	Heavy metal speciation is the analytical and geochemical determination of the chemical forms (species) and partitioning of toxic metals (lead, cadmium, chromium, zinc, copper) in soil, sediment, and water. Metal bioavailability—the fraction accessible to organisms—depends critically on speciation: metal bound to soil organic matter or iron oxides is immobile and non-bioavailable; dissolved or exchangeable metal is highly bioavailable and toxic. Speciation assessment informs remediation design, risk assessment, and contaminant fate prediction.	Ecotoxicological testing is a suite of standardized laboratory and field methods to assess the toxicity of chemical substances to aquatic and terrestrial organisms (fish, invertebrates, algae, plants, soil fauna). Developed by regulatory agencies (OECD, EPA, EMEA) since the 1970s, these tests measure lethal concentration (LC50, EC50) and sublethal endpoints (growth, reproduction, behavior) under controlled conditions. Ecotoxicological data support chemical hazard classification, environmental risk assessment, and regulatory approval of new substances.