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| Yhden solun geenijoukkojen rikastumisanalyysi× | Yksittäisen solun RNA-sekvensointi -analyysi× | |
|---|---|---|
| Tieteenala | Bioinformatiikka | Bioinformatiikka |
| Menetelmäperhe | Process / pipeline | Process / pipeline |
| Syntyvuosi≠ | 2017-2019 | 2009 (first scRNA-seq by Tang et al.); widely adopted 2015–2016 |
| Kehittäjä≠ | Sara Aibar, Stein Aerts (AUCell/SCENIC); David DeTomaso, Nir Yosef (VISION) | Azim Surani, Barbara Treutlein, and the Regev/McCarroll groups (foundational droplet-based methods ~2015) |
| Tyyppi≠ | Computational enrichment scoring pipeline | High-throughput single-cell transcriptomic profiling pipeline |
| Alkuperäislähde≠ | Aibar, S., Gonzalez-Blas, C. B., Moerman, T., Huynh-Thu, V. A., Imrichova, H., Hulselmans, G., Rambow, F., Marine, J.-C., Geurts, P., Aerts, J., van den Oord, J., Kalender Atak, Z., Wouters, J., & Aerts, S. (2017). SCENIC: Single-cell regulatory network inference and clustering. Nature Methods, 14(11), 1083-1086. link ↗ | Satija, R., Farrell, J. A., Gennert, D., Schier, A. F., & Regev, A. (2015). Spatial reconstruction of single-cell gene expression data. Nature Biotechnology, 33(5), 495–502. DOI ↗ |
| Rinnakkaisnimet | scGSEA, single-cell GSEA, cell-level gene set scoring, scRNA-seq pathway scoring | scRNA-seq, single-cell transcriptomics, scRNAseq analysis, single-cell gene expression profiling |
| Liittyvät | 5 | 5 |
| Tiivistelmä≠ | Single-cell gene set enrichment analysis (scGSEA) extends classical bulk GSEA to the resolution of individual cells. Rather than testing whether a gene set is enriched in a sample-level comparison, scGSEA assigns an enrichment or activity score to each cell, enabling researchers to map pathway activity across heterogeneous cell populations, cell states, and developmental trajectories captured in single-cell RNA-seq data. | Single-cell RNA sequencing (scRNA-seq) analysis characterises gene expression at the resolution of individual cells, enabling discovery of cell types, states, and transitions that are invisible in bulk transcriptomics. Starting from raw sequencing reads, the workflow produces a cell-by-gene count matrix and proceeds through quality control, normalisation, dimensionality reduction, unsupervised clustering, cell-type annotation, and a range of downstream analyses such as trajectory inference and differential expression between cell populations. |
| ScholarGateAineisto ↗ |
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