Vertaile menetelmiä

	Hi-C-analyysi	RNA-nopeus
Tieteenala	Genetiikka	Genetiikka
Menetelmäperhe	Process / pipeline	Process / pipeline
Syntyvuosi	2009	2018
Kehittäjä	Erez Lieberman-Aiden & Job Dekker	Gioele La Manno & Pavel Soldatov
Tyyppi	Chromatin interaction method	Trajectory inference method
Alkuperäislähde	Lieberman-Aiden, E., van Berkum, N. L., Williams, L., Imakaev, M., Ragoczy, T., Telling, A., & Dekker, J. (2009). Comprehensive mapping of long-range interactions reveals folding principles of the human genome. Science, 326(5950), 289–293.	La Manno, G., Soldatov, R., Zeisel, A., Braun, E., Hochgerner, H., Petukhov, V., & Merad, M. (2018). RNA velocity of single cells. Nature, 560(7737), 494–498.
Rinnakkaisnimet	Chromosome conformation capture, 3D genome, Chromatin contact mapping	Velocity analysis, Transcriptomic velocity, Cell fate prediction
Liittyvät	2	2
Tiivistelmä	Hi-C (High-Chromosome Conformation Capture) is a technique and associated computational methods for mapping the 3D architecture of the genome within cells. Developed by Lieberman-Aiden and Dekker in 2009, Hi-C identifies physical interactions between genomic regions that may be distant in linear sequence but spatially proximal in 3D nuclear space. Hi-C analysis has revealed fundamental principles of genome organization, including the existence of topologically associating domains (TADs), and provides insights into how 3D structure regulates gene expression and DNA replication.	RNA velocity is a computational method that infers the future developmental state of individual cells from single-cell RNA-sequencing data. Developed by La Manno and colleagues in 2018, RNA velocity analysis measures the direction and pace of cell state transitions by analyzing the ratio of unspliced to spliced mRNA transcripts within individual cells. This enables prediction of cell trajectories and differentiation pathways without requiring temporal sampling or manipulation, providing unique insights into cell fate decisions during development and disease.