Klason Lignin

The Klason lignin method is a standard chemical test for quantifying the acid-insoluble lignin content in wood and plant biomass. Developed by Erik Klason in 1908, the method treats wood with sulfuric acid to dissolve carbohydrates (cellulose and hemicellulose) while leaving the acid-insoluble lignin residue. Klason lignin is widely used in wood science, pulp chemistry, and biomass characterization to assess wood composition and predict properties.