Process / pipelineRadical scavenging / antioxidant capacity
DPPH Radical Scavenging Assay — Antioxidant Capacity Measurement
The DPPH radical scavenging assay is a rapid, widely used spectrophotometric method for measuring the antioxidant capacity of foods, plant extracts, and purified compounds. It quantifies how effectively a sample neutralises the stable synthetic free radical DPPH (2,2-diphenyl-1-picrylhydrazyl) by measuring the resulting colour change from deep violet to yellow, making it a cornerstone technique in food science, nutraceutical research, and phytochemistry.
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Sources
- Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199–1200. DOI: 10.1038/1811199a0 ↗
- Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT — Food Science and Technology, 28(1), 25–30. DOI: 10.1016/S0023-6438(95)80008-5 ↗