Hemagglutination Inhibition Assay
The Hemagglutination Inhibition (HI) Assay is a classical serological test used to detect and quantify antibodies against hemagglutinating viruses — most notably influenza and Newcastle disease virus — in animal and human serum. Widely employed in veterinary diagnostics, vaccine efficacy evaluation, and epidemiological surveillance, it relies on the principle that specific antibodies in serum will block a known quantity of virus from agglutinating red blood cells.
Source record
Citations copied verbatim from the method’s source record. No claim-level verification is inferred from them.
- Hirst, G. K. (1942). The quantitative determination of influenza virus and antibodies by means of red cell agglutination. Journal of Experimental Medicine, 75(1), 49–64. · DOI 10.1084/jem.75.1.49
- World Organisation for Animal Health (WOAH/OIE). (2021). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 3.3.4 (Avian Influenza). WOAH. · URL
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