Methoden vergleichen

	Analyse der Diversität von Mikrobiomen mittels Multi-Omics-Ansätzen	Pathway Enrichment Analysis
Fachgebiet	Bioinformatik	Bioinformatik
Familie	Process / pipeline	Process / pipeline
Entstehungsjahr	2010s–present	2003–2005
Urheber	Developed collectively; key frameworks by Le Cao et al. (mixOmics, 2017) and Argelaguet et al. (MOFA, 2018)	Mootha et al. (2003); systematised by Subramanian et al. (2005)
Typ	Integrative computational pipeline	Statistical functional annotation method
Wegweisende Quelle	Rohart, F., Gautier, B., Singh, A., & Le Cao, K.-A. (2017). mixOmics: An R package for 'omics feature selection and multiple data integration. PLOS Computational Biology, 13(11), e1005752.	Subramanian, A., Tamayo, P., Mootha, V. K., Mukherjee, S., Ebert, B. L., Gillette, M. A., Paulovich, A., Pomeroy, S. L., Golub, T. R., Lander, E. S., & Mesirov, J. P. (2005). Gene set enrichment analysis: A knowledge-based approach for interpreting genome-wide expression profiles. Proceedings of the National Academy of Sciences, 102(43), 15545–15550.
Aliasnamen	multi-omics microbiome profiling, integrated microbiome omics, multi-modal microbiome analysis, microbiome multi-omics integration	PEA, overrepresentation analysis, ORA, functional enrichment analysis
Verwandt	5	6
Zusammenfassung	Multi-omics microbiome diversity analysis integrates two or more omic data layers — such as metagenomics, metatranscriptomics, metabolomics, and metaproteomics — to characterise both the composition and functional activity of microbial communities. By linking taxonomic diversity metrics with molecular phenotype data, the approach uncovers how community structure translates into ecological and host-relevant functions that no single omic layer can reveal alone.	Pathway enrichment analysis (PEA) is a statistical approach that takes a list of genes or proteins of interest — typically derived from a differential expression or proteomics experiment — and identifies which pre-defined biological pathways or functional gene sets are represented more often than expected by chance. By mapping individual molecular changes onto curated pathway knowledge bases such as KEGG, Gene Ontology, or Reactome, PEA translates long gene lists into interpretable biological processes, making it a central tool in the post-analysis of high-throughput omics experiments.