Сравнение на методи

	Подравняване на последователности	RNA-seq анализ на диференциална експресия
Област	Биоинформатика	Биоинформатика
Семейство	Process / pipeline	Process / pipeline
Година на възникване	1970 (global alignment); 1981 (local alignment)	2008–2010 (RNA-seq DE methodology established)
Създател	Saul B. Needleman & Christian D. Wunsch (global); Temple F. Smith & Michael S. Waterman (local)	Multiple groups; foundational methods from Anders & Huber (DESeq, 2010), Robinson, McCarthy & Smyth (edgeR, 2010)
Тип	Computational sequence analysis technique	Quantitative genomics pipeline
Основополагащ източник	Needleman, S. B., & Wunsch, C. D. (1970). A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology, 48(3), 443–453.	Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12), 550.
Други названия	pairwise alignment, multiple sequence alignment, MSA, sequence comparison	RNA-seq DE analysis, transcriptomic differential expression, bulk RNA-seq DE, DEA
Свързани	6	6
Резюме	Sequence alignment is a foundational bioinformatics technique that arranges two or more DNA, RNA, or protein sequences to reveal regions of similarity, infer evolutionary relationships, identify functional domains, and map sequencing reads to reference genomes. It underpins virtually every downstream genomic analysis, from variant calling and gene expression quantification to phylogenetics and structural annotation.	RNA-seq differential expression (DE) analysis identifies genes whose transcript abundance differs significantly between two or more biological conditions — for example, treated versus control, or diseased versus healthy tissue. Starting from raw sequencing reads, the pipeline moves through alignment, count-based normalization, statistical modeling of count dispersion, hypothesis testing, and multiple-testing correction to produce a ranked list of differentially expressed genes accompanied by fold-change estimates and adjusted p-values.