方法证据记录
Isothermal Titration Calorimetry
Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization.
源记录
引文逐字复制自方法源记录。这些引文不代表任何层级的验证。
Isothermal Titration Calorimetry
分类方法记录 · process-pipeline / spectroscopy
- Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. · DOI 10.1016/0003-2697(89)90213-3
- Garbett, N. C., & Chaires, J. B. (2012). Binding: A statistical thermodynamic model. Biophysical Journal, 86(6), 3493-3494. · URL
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