So sánh phương pháp

	NOESY	HSQC
Lĩnh vực	Quang phổ học	Quang phổ học
Họ	Process / pipeline	Process / pipeline
Năm ra đời	1981	1980
Người khởi xướng	Richard Ernst	Anil Kumar
Loại	Two-dimensional pulse sequence	Heteronuclear correlation sequence
Công trình gốc	Aue, W. P., Bartholdi, E., & Ernst, R. R. (1976). Two-dimensional spectroscopy. Application to nuclear magnetic resonance. The Journal of Chemical Physics, 64(5), 2229-2246.	Bodenhausen, G., & Ruben, D. J. (1981). Natural abundance nitrogen-15 NMR by enhanced heteronuclear spectroscopy. Chemical Physics Letters, 69(2), 185-189.
Tên gọi khác	NOE spectroscopy, 2D NOESY, NOE NMR	HSQC NMR, 1H-13C HSQC, heteronuclear correlation
Liên quan	3	4
Tóm tắt	Nuclear Overhauser Enhancement Spectroscopy (NOESY) is a 2D NMR technique that detects through-space dipolar coupling between protons, rather than through-bond scalar coupling. Introduced by Macura and Ernst in 1981, NOESY reveals which protons are spatially close in the three-dimensional structure, independent of bonding connectivity. This makes NOESY invaluable for determining molecular conformation, assigning stereochemistry, and elucidating protein folds.	Heteronuclear Single-Quantum Coherence (HSQC) is a 2D NMR technique that correlates proton and carbon-13 (or other heteronuclei) chemical shifts through one-bond coupling constants (1JHX). Developed in the early 1980s, HSQC rapidly became the workhorse of structural chemistry because it directly maps which carbons bear which protons, providing a comprehensive view of carbon skeleton connectivity and substitution patterns.