Jämför metoder
Granska de valda metoderna sida vid sida; rader som skiljer sig är markerade.
| Transwell-analys× | MTT/MTS-analysen× | |
|---|---|---|
| Ämnesområde | Biomaterial | Biomaterial |
| Familj | Process / pipeline | Process / pipeline |
| Ursprungsår≠ | 1962 | 1983 |
| Upphovsperson≠ | Stephen Boyden | Tatsuro Mosmann |
| Typ≠ | Migration/invasion assay | Colorimetric assay |
| Ursprungskälla≠ | Boyden, S. (1962). The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. Journal of Experimental Medicine, 115(3), 453-466. DOI ↗ | Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. DOI ↗ |
| Alias | Boyden chamber assay, chemotaxis assay, invasion chamber assay | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay |
| Närliggande | 4 | 4 |
| Sammanfattning≠ | The Transwell assay (also called the Boyden chamber assay after its originator Stephen Boyden) is a quantitative method for measuring cell migration and invasion in response to chemical gradients or through matrix barriers. The assay uses a membrane insert with defined pore size suspended in a multi-well plate: cells are placed in the upper chamber, a chemoattractant is placed in the lower chamber, and cells that successfully migrate through the pores accumulate in the lower chamber, where they can be counted or visualized. Variants that coat the insert with matrix proteins (Matrigel, collagen) enable measurement of invasion capacity. The Transwell assay is a gold-standard method in cell biology for evaluating cell motility, tumor metastatic potential, and the effects of growth factors and inhibitory compounds. | The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development. |
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