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| Nätverksbaserad encells-RNA-sekvensanalys× | Single-cell eQTL-analys× | |
|---|---|---|
| Ämnesområde | Bioinformatik | Bioinformatik |
| Familj | Process / pipeline | Process / pipeline |
| Ursprungsår≠ | 2015–2017 (rapid development alongside scRNA-seq methods; SCENIC 2017) | 2020 |
| Upphovsperson≠ | Aibar et al. (SCENIC, gene regulatory networks); Jin et al. (CellChat, cell-cell communication networks) | Cuomo et al.; Kim-Hellmuth et al. (pioneering sc-eQTL frameworks, 2020) |
| Typ≠ | Computational bioinformatics pipeline | Statistical genomics pipeline |
| Ursprungskälla≠ | Aibar, S., González-Blas, C. B., Moerman, T., Huynh-Thu, V. A., Imrichova, H., Hulselmans, G., ... & Aerts, S. (2017). SCENIC: single-cell regulatory network inference and clustering. Nature Methods, 14(11), 1083–1086. link ↗ | Cuomo, A. S. E., et al. (2020). Single-cell RNA-sequencing of differentiating iPS cells reveals dynamic genetic effects on gene expression. Nature Communications, 11(1), 810. link ↗ |
| Alias | scRNA-seq network analysis, single-cell gene regulatory network inference, scGRN analysis, single-cell co-expression network analysis | sc-eQTL analysis, single-cell eQTL mapping, scRNA-seq eQTL, cell-type-specific eQTL |
| Närliggande | 6 | 6 |
| Sammanfattning≠ | Network-based single-cell RNA-seq analysis extends standard scRNA-seq workflows by constructing and interrogating molecular interaction networks — gene regulatory networks, co-expression networks, or cell-cell communication graphs — from single-cell transcriptomic data. Rather than treating each gene independently, this approach captures the coordinated activity of gene circuits and intercellular signalling pathways within and between cell populations, enabling a systems-level view of transcriptional regulation at single-cell resolution. | Single-cell eQTL analysis identifies genetic variants (eQTLs) that regulate gene expression in a cell-type-specific manner by jointly analysing single-cell RNA-seq profiles and donor genotype data. Unlike bulk eQTL methods, it resolves regulatory effects that are diluted or masked when cell types are mixed, enabling discovery of variants whose effects are confined to particular cell states or developmental stages. |
| ScholarGateDatamängd ↗ |
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