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| Alizarinfärgning× | MTT/MTS-analysen× | |
|---|---|---|
| Ämnesområde | Biomaterial | Biomaterial |
| Familj | Process / pipeline | Process / pipeline |
| Ursprungsår≠ | 2004 | 1983 |
| Upphovsperson≠ | Gregory, Gunn, Peister | Tatsuro Mosmann |
| Typ≠ | Staining assay | Colorimetric assay |
| Ursprungskälla≠ | Gregory, C. A., Gunn, W. G., Peister, A., & Prockop, D. J. (2004). An Alizarin red-based assay of mineralization by adherent cells in culture: comparison with cetylpyridinium chloride extraction. Analytical Biochemistry, 329(1), 77-84. DOI ↗ | Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. DOI ↗ |
| Alias | alizarin red-S, calcium staining, bone mineralization assay | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay |
| Närliggande | 4 | 4 |
| Sammanfattning≠ | Alizarin red-S (1,2-dihydroxyanthraquinone-3-sulfonic acid) is a calcium-binding dye that forms a colored complex with mineralized deposits, enabling direct visualization and quantification of bone matrix mineralization. Developed as a standard assay by Gregory and colleagues in 2004, alizarin red staining is widely used to evaluate osteogenic differentiation of stem cells, assess the mineralization-promoting effects of biomaterial scaffolds and growth factors, and measure the calcium content of bone tissue and engineered constructs. The assay is rapid, quantitative, and provides both visual and colorimetric readout. | The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development. |
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