Krahasoni metodat

	Citometria e Masës Imazherike	Ultratingullimi Funksional
Fusha	Imazheria mjekësore	Imazheria mjekësore
Familja	Process / pipeline	Process / pipeline
Viti i origjinës	2014	2011
Krijuesi	Bernd Bodenmiller	Mickael Tanter
Lloji	Multiplexed single-cell imaging by mass spectrometry	High-framerate doppler imaging for hemodynamics
Burimi themelues	Giesen, C., Wang, H. A., Schapiro, D., et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11(4), 417-422.	Macé, E., Montaldo, G., Trenholm, S., et al. (2011). Functional ultrasound imaging of the brain. Nature Methods, 8(8), 662-664.
Emërtime të tjera	IMC, mass cytometry, multiplex ion beam imaging, MIBI	fUS, doppler ultrasound, ultrafast ultrasound
Të lidhura	5	5
Përmbledhja	Imaging Mass Cytometry (IMC) is a multiplexed proteomics technique that maps the subcellular localization of up to 40-50 proteins in tissue sections simultaneously using mass spectrometry detection. Developed by Bodenmiller and colleagues in 2014, IMC combines the single-cell imaging power of immunofluorescence with the multiplexing capacity of mass cytometry, enabling comprehensive analysis of cell types, states, and spatial interactions within tissue microenvironments. IMC has emerged as a powerful tool in immuno-oncology, immunobiology, and tissue biology for dissecting cellular heterogeneity and spatial organization.	Functional Ultrasound (fUS) is a high-framerate Doppler ultrasound technique that dynamically maps blood flow and hemodynamic changes in vivo with millisecond temporal resolution. Pioneered by Tanter, Macé, and colleagues in the 2010s, fUS enables real-time imaging of microvascular perfusion in the brain and other organs. By combining ultrafast acquisition (1000-5000 frames per second) with Doppler processing, fUS reveals functional activity (hemodynamic changes during stimulation or behavior) and vascular networks with unprecedented spatiotemporal detail.