Krahasoni metodat

	Modelimi i Homologjisë	Rikthimi me Krioelektron Mikroskopi (Cryo-EM)
Fusha	Bioinformatikë	Bioinformatikë
Familja	Process / pipeline	Process / pipeline
Viti i origjinës	1993	1975
Krijuesi	Andrej Sali	Joachim Frank
Lloji	Comparative structure prediction pipeline	Image reconstruction pipeline
Burimi themelues	Sali, A. & Blundell, T. L. (1993). Comparative protein modelling by satisfaction of spatial restraints. Journal of Molecular Biology, 234(3), 779-815.	Frank, J. (2002). Single-particle imaging of macromolecules by cryo-electron microscopy. Annual Review of Biophysics and Biomolecular Structure, 31, 303-319.
Emërtime të tjera	comparative modeling, template-based modeling	cryo-electron microscopy, cryo-EM, single-particle cryo-EM
Të lidhura	4	3
Përmbledhja	Homology modeling, also called comparative modeling, predicts the three-dimensional structure of a protein using an experimentally-solved structure of a homologous protein as a template. Introduced by Sali and Blundell in 1993, this method exploits the principle that homologous proteins share similar spatial structures despite differing in amino acid sequence.	Cryo-electron microscopy (cryo-EM) determines three-dimensional macromolecular structures at atomic or near-atomic resolution by imaging proteins frozen in vitreous ice. Pioneered by Frank, Henderson, and others, this technique has revolutionized structural biology by enabling visualization of large, non-crystallizable complexes and capturing functional conformational states.