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Клеточный тест на проницаемость Caco-2×Фактор подобия растворения f1/f2×
ОбластьФармакологияФармакология
СемействоProcess / pipelineProcess / pipeline
Год появления19891996
Автор методаIngrid HidalgoJames Moore and Hector Flanner
Типabsorption screeningsimilarity testing
Основополагающий источникHidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749. DOI ↗Moore, J. W., & Flanner, H. H. (1996). Mathematical comparison of dissolution profiles. Pharmaceutical Technology, 20(6), 64-74. link ↗
Другие названияCaco-2 assay, intestinal permeability, ADME screeningf1, f2, similarity factor
Связанные33
СводкаThe Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.The f1 and f2 factors are dimensionless statistical measures developed by Moore and Flanner to quantify the similarity between two dissolution profiles. Adopted by regulatory agencies (FDA, EMA) as the gold standard for comparing dissolution curves, these factors enable rapid assessment of whether formulation changes significantly impact drug release.
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  2. 2 Источники
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ScholarGateСравнение методов: Caco-2 Permeability · Dissolution f1/f2 Similarity. Получено 2026-06-20 из https://scholargate.app/ru/compare