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Test przepuszczalności komórek Caco-2×Współczynnik podobieństwa rozpadu f1/f2×
DziedzinaFarmakologiaFarmakologia
RodzinaProcess / pipelineProcess / pipeline
Rok powstania19891996
TwórcaIngrid HidalgoJames Moore and Hector Flanner
Typabsorption screeningsimilarity testing
Źródło pierwotneHidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749. DOI ↗Moore, J. W., & Flanner, H. H. (1996). Mathematical comparison of dissolution profiles. Pharmaceutical Technology, 20(6), 64-74. link ↗
Inne nazwyCaco-2 assay, intestinal permeability, ADME screeningf1, f2, similarity factor
Pokrewne33
PodsumowanieThe Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.The f1 and f2 factors are dimensionless statistical measures developed by Moore and Flanner to quantify the similarity between two dissolution profiles. Adopted by regulatory agencies (FDA, EMA) as the gold standard for comparing dissolution curves, these factors enable rapid assessment of whether formulation changes significantly impact drug release.
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ScholarGatePorównaj metody: Caco-2 Permeability · Dissolution f1/f2 Similarity. Pobrano 2026-06-19 z https://scholargate.app/pl/compare