Methoden vergelijken

	Differentiële Metabolomics Analyse	RNA-seq Differentiele Expressie
Vakgebied	Bio-informatica	Bio-informatica
Familie	Process / pipeline	Process / pipeline
Jaar van ontstaan	2000s–2010s (field formalised alongside mass spectrometry advances)	2008–2010 (RNA-seq DE methodology established)
Grondlegger	Developed through convergent contributions by multiple groups; XCMS (Siuzdak lab, 2006) and MetaboAnalyst (Wishart lab, 2009–2015) are foundational computational implementations	Multiple groups; foundational methods from Anders & Huber (DESeq, 2010), Robinson, McCarthy & Smyth (edgeR, 2010)
Type	Quantitative comparative omics pipeline	Quantitative genomics pipeline
Oorspronkelijke bron	Xia, J., Sinelnikov, I. V., Han, B., & Wishart, D. S. (2015). MetaboAnalyst 3.0 — making metabolomics more meaningful. Nucleic Acids Research, 43(W1), W251–W257.	Love, M. I., Huber, W., & Anders, S. (2014). Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biology, 15(12), 550.
Aliassen	comparative metabolomics, differential metabolite profiling, metabolomic differential analysis, DMA	RNA-seq DE analysis, transcriptomic differential expression, bulk RNA-seq DE, DEA
Verwant	6	6
Samenvatting	Differential metabolomics analysis is a computational pipeline that identifies metabolites whose abundance levels differ significantly between two or more biological conditions — such as disease versus control, treated versus untreated, or different developmental stages. By integrating mass spectrometry or NMR data with statistical modelling and pathway databases, it translates raw spectral measurements into biologically interpretable lists of perturbed metabolic features and the pathways they implicate.	RNA-seq differential expression (DE) analysis identifies genes whose transcript abundance differs significantly between two or more biological conditions — for example, treated versus control, or diseased versus healthy tissue. Starting from raw sequencing reads, the pipeline moves through alignment, count-based normalization, statistical modeling of count dispersion, hypothesis testing, and multiple-testing correction to produce a ranked list of differentially expressed genes accompanied by fold-change estimates and adjusted p-values.