Methoden vergelijken
Bekijk de geselecteerde methoden naast elkaar; rijen die verschillen zijn gemarkeerd.
| Chronoamperometrie× | Surface Plasmon Resonance× | |
|---|---|---|
| Vakgebied | Spectroscopie | Spectroscopie |
| Familie | Process / pipeline | Process / pipeline |
| Jaar van ontstaan≠ | 1954 | 1971 |
| Grondlegger≠ | Paul Delahay | Erich Kretschmann |
| Type≠ | Electrochemical technique | Optical technique |
| Oorspronkelijke bron≠ | Bard, A. J., & Faulkner, L. R. (2001). Electrochemical Methods: Fundamentals and Applications. John Wiley & Sons, 2nd edition. link ↗ | Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324. link ↗ |
| Aliassen≠ | CA, chronoamperometric method | SPR, surface plasmon, SPR biosensing |
| Verwant | 3 | 3 |
| Samenvatting≠ | Chronoamperometry (CA) is an electrochemical technique that measures current as a function of time when a potential step is applied to an electrode. Developed by Delahay in the 1950s, CA reveals diffusion-controlled electrochemical processes and enables determination of diffusion coefficients, surface coverage, and kinetic rate constants by analyzing the transient current decay. | Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery. |
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