विधियों की तुलना करें
चुनी हुई विधियों की आमने-सामने समीक्षा करें; भिन्नता वाली पंक्तियाँ रेखांकित हैं।
| ऊष्माशोषी अनुमापन कैलोरीमिति× | इलेक्ट्रॉन पैरामैग्नेटिक अनुनाद× | |
|---|---|---|
| क्षेत्र | स्पेक्ट्रोस्कोपी | स्पेक्ट्रोस्कोपी |
| परिवार | Process / pipeline | Process / pipeline |
| उद्भव वर्ष≠ | 1989 | 1945 |
| प्रवर्तक≠ | Terrence Wiseman | Evgeny Zavoiskii |
| प्रकार≠ | Biophysical technique | Spectroscopic technique |
| मौलिक स्रोत≠ | Wiseman, T., Williston, S., Brandts, J. F., & Lin, L. N. (1989). Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Analytical Biochemistry, 179(1), 131-137. DOI ↗ | Zavoiskii, E. K. (1945). Paramagnetic relaxation of liquid solutions for perpendicular fields. Zhurnal Eksperimental'noi i Teoreticheskoi Fiziki, 15(6), 378-380. link ↗ |
| उपनाम | ITC, isothermal calorimetry, microcalorimetry | EPR spectroscopy, ESR, electron spin resonance |
| संबंधित | 3 | 3 |
| सारांश≠ | Isothermal Titration Calorimetry (ITC) is a thermodynamic technique that measures heat released or absorbed during biomolecular binding events at constant temperature. Developed by Wiseman and colleagues in 1989, ITC directly determines binding affinity (Kd), enthalpy (ΔH), and entropy (ΔS) in a single experiment, making it one of the most comprehensive methods for characterizing molecular interactions without requiring labels or immobilization. | Electron Paramagnetic Resonance (EPR), also called Electron Spin Resonance (ESR), is a spectroscopic technique that detects and characterizes unpaired electrons in molecules and materials. Discovered by Zavoiskii in 1945, EPR measures the absorption of microwave radiation by paramagnetic species in a magnetic field, providing information about electron spin states, local electronic environment, and molecular dynamics. |
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