השוואת שיטות

	אלקטרופיזיולוגיה בשיטת Patch-Clamp	ניתוח שילד
תחום	פרמקולוגיה	פרמקולוגיה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	1976	1947
הוגה השיטה	Erwin Neher and Bert Sakmann	Henry Schild
סוג	ion channel screening	antagonism quantification
מקור מכונן	Neher, E., & Sakmann, B. (1976). Single-channel currents recorded from membrane of denervated frog muscle fibres. Nature, 260(5554), 799-802.	Schild, H. O. (1947). pA, a new scale for the measurement of drug antagonism. Journal of Physiology, 106(3), 337-357.
כינויים	patch clamp, whole-cell recording, ion channel assay	Schild plot, pA2
קשורות	3	3
תקציר	Patch-clamp electrophysiology is a technique for measuring ionic currents through ion channels in cell membranes, developed by Neher and Sakmann in 1976. It enables direct observation of single-channel and whole-cell currents at millisecond resolution, making it essential for characterizing drug effects on ion channels and cardiac safety assessment.	Schild analysis is a quantitative method for characterizing competitive receptor antagonism developed by Henry Schild in 1947. It uses dose-response curves in the presence and absence of antagonist to estimate the antagonist affinity constant (pA2), enabling standardized comparison of antagonist potency across drugs and experimental systems.