השוואת שיטות

	CAM Assay	מבחן שריטת פצע (Scratch Wound Assay)
תחום	ביו-חומרים	ביו-חומרים
משפחה	Process / pipeline	Process / pipeline
שנת המקור	1974	2007
הוגה השיטה	Judah Folkman	Liang, Park, and Guan
סוג	Developmental biology assay	Migration assessment
מקור מכונן	Folkman, J. (1974). Tumor angiogenesis: therapeutic implications. New England Journal of Medicine, 285(21), 1182-1186.	Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333.
כינויים	chick embryo chorioallantoic membrane, angiogenesis assay, CAM angiogenesis model	wound healing assay, gap closure assay, migration assay
קשורות	4	4
תקציר	The chorioallantoic membrane (CAM) assay is a well-established in vivo model for studying angiogenesis (new blood vessel formation) and evaluating the pro- or anti-angiogenic properties of biomaterials, drugs, and bioactive molecules. Developed by Judah Folkman in the 1970s, the assay uses the highly vascularized CAM of developing chick embryos as a platform for implanting test materials and observing vascular response. The CAM provides a transparent, immunologically naive microenvironment with rapid and reproducible neovascularization, making it ideal for screening angiogenic potential and assessing biomaterial biocompatibility.	The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential.