השוואת שיטות

	מבחן חדירות תאי Caco-2	גורם דמיון f1/f2 (Dissolution f1/f2 Similarity Factor)
תחום	פרמקולוגיה	פרמקולוגיה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	1989	1996
הוגה השיטה	Ingrid Hidalgo	James Moore and Hector Flanner
סוג	absorption screening	similarity testing
מקור מכונן	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.	Moore, J. W., & Flanner, H. H. (1996). Mathematical comparison of dissolution profiles. Pharmaceutical Technology, 20(6), 64-74.
כינויים	Caco-2 assay, intestinal permeability, ADME screening	f1, f2, similarity factor
קשורות	3	3
תקציר	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.	The f1 and f2 factors are dimensionless statistical measures developed by Moore and Flanner to quantify the similarity between two dissolution profiles. Adopted by regulatory agencies (FDA, EMA) as the gold standard for comparing dissolution curves, these factors enable rapid assessment of whether formulation changes significantly impact drug release.