השוואת שיטות

	Ancient DNA Analysis	Stable Isotope Paleodiet & Mobility Analysis
תחום	ארכאולוגיה	ארכאולוגיה
משפחה	Process / pipeline	Process / pipeline
שנת המקור	2004	2006
הוגה השיטה	Svante Paabo and colleagues (foundational methodology)	Synthesis of stable-isotope bioarchaeology; strontium methodology reviewed by R. Alexander Bentley
סוג	Laboratory and computational pipeline for recovering and authenticating genetic data from archaeological remains	Methodological pipeline for trophic, dietary-source, and mobility inference from skeletal isotopes
מקור מכונן	Paabo, S., et al. (2004). Genetic Analyses from Ancient DNA. Annual Review of Genetics, 38, 645-679.	Bentley, R. A. (2006). Strontium Isotopes from the Earth to the Archaeological Skeleton: A Review. Journal of Archaeological Method and Theory, 13(3), 135-187.
כינויים	aDNA Analysis, Archaeogenetics, Ancient Genomics, Palaeogenetics	Carbon-Nitrogen Collagen Isotope Analysis, Trophic Spacing Analysis, Bioarchaeological Isotope Methodology, Collagen Quality Screening
קשורות	2	2
תקציר	Ancient DNA analysis recovers genetic information from the degraded remains of past organisms — human and animal bones and teeth, and increasingly sediments — and uses it to reconstruct kinship, ancestry, population history, sex, pathogens, and domestication. Because DNA fragments into ever-shorter pieces and accumulates characteristic chemical damage after death, and because a handful of modern molecules can swamp the few authentic ones, the field is defined less by sequencing itself than by an exacting protocol of clean-lab extraction, contamination control, and authentication. The foundational reviews by Svante Paabo and colleagues set out the principles that distinguish genuine ancient sequences from contaminants, and the move to next-generation sequencing transformed aDNA from a fragile curiosity into a routine source of genome-scale data.	Stable isotope paleodiet and mobility analysis is the methodology by which bioarchaeologists turn the isotopic chemistry of bone and tooth into quantitative statements about what people ate and where they lived. It rests on a chain of disciplined procedures rather than a single measurement: screening extracted collagen for diagenetic integrity using carbon-to-nitrogen atomic ratios, anchoring human values to a locally measured faunal baseline, quantifying trophic position from nitrogen-15 spacing, partitioning C3 versus C4 and marine carbon sources using the offset between collagen and apatite, and reconstructing residential mobility from biologically available strontium isotopes in tooth enamel. Bentley's review of strontium in the archaeological skeleton and Evershed's account of the biomarker revolution together frame the geochemical and analytical principles that make these inferences defensible.