Vertaile menetelmiä
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| ATR-FTIR× | Plasmoniresonanssi pinnassa× | |
|---|---|---|
| Tieteenala | Spektroskopia | Spektroskopia |
| Menetelmäperhe | Process / pipeline | Process / pipeline |
| Syntyvuosi≠ | 1961 | 1971 |
| Kehittäjä≠ | Joop Fahrenfort | Erich Kretschmann |
| Tyyppi≠ | Vibrational spectroscopy technique | Optical technique |
| Alkuperäislähde≠ | Harrick, N. J. (1960). Study of physics of internal reflection from metals. Journal of Physics and Chemistry of Solids, 13(2), 143-155. link ↗ | Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324. link ↗ |
| Rinnakkaisnimet | ATR-IR, attenuated total reflectance, FTIR spectroscopy | SPR, surface plasmon, SPR biosensing |
| Liittyvät | 3 | 3 |
| Tiivistelmä≠ | Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) spectroscopy is a variant of conventional FTIR that measures infrared absorption through evanescent-wave interrogation of samples in direct contact with a high-refractive-index crystal. Developed by Harrick and Fahrenfort in the 1960s, ATR-FTIR is now the dominant form of FTIR spectroscopy, enabling rapid, non-destructive characterization of organic compounds, polymers, coatings, and biological materials without extensive sample preparation. | Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery. |
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