مقایسهٔ روش‌ها

	تحلیل فلوسایتومتری	آزمایش نفوذپذیری سلول Caco-2
حوزه	داروشناسی	داروشناسی
خانواده	Process / pipeline	Process / pipeline
سال پیدایش	1976	1989
پدیدآور	Leonard Herzenberg	Ingrid Hidalgo
نوع	cell analysis and sorting	absorption screening
منبع بنیادین	Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827.	Hidalgo, I. J., Raub, T. J., & Borchardt, R. T. (1989). Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96(3), 736-749.
نام‌های دیگر	FACS, fluorescence-activated cell sorting, cell analysis	Caco-2 assay, intestinal permeability, ADME screening
مرتبط	3	3
خلاصه	Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology.	The Caco-2 assay is an in vitro model system using human colon carcinoma cell monolayers to screen drug intestinal permeability. Developed by Hidalgo and colleagues in 1989, Caco-2 cells differentiate into an epithelial barrier resembling intestinal mucosa, enabling rapid assessment of drug absorption potential and identification of transporter-mediated transport.