مقایسهٔ روش‌ها

	آزمون‌های اکوتوکسیکولوژی	تعیین ماهیت فلزات سنگین
حوزه	مهندسی محیط زیست	مهندسی محیط زیست
خانواده	Process / pipeline	Process / pipeline
سال پیدایش	1975	1979
پدیدآور	EPA and OECD	Tessier and hydrogeochemists
نوع	experimental measurement and analysis pipeline	analytical and geochemical modeling pipeline
منبع بنیادین	OECD. (2011). Test Guidelines for Chemicals. OECD Publishing.	Tessier, A., Campbell, P. G. C., & Bisson, M. (1979). Sequential Extraction Procedure for the Speciation of Particulate Trace Metals. Analytical Chemistry, 51(7), 844–851.
نام‌های دیگر	toxicity testing, aquatic bioassay, ecotoxicity assessment, organism exposure testing	metal speciation, metal partitioning, bioavailability assessment, speciation analysis
مرتبط	3	3
خلاصه	Ecotoxicological testing is a suite of standardized laboratory and field methods to assess the toxicity of chemical substances to aquatic and terrestrial organisms (fish, invertebrates, algae, plants, soil fauna). Developed by regulatory agencies (OECD, EPA, EMEA) since the 1970s, these tests measure lethal concentration (LC50, EC50) and sublethal endpoints (growth, reproduction, behavior) under controlled conditions. Ecotoxicological data support chemical hazard classification, environmental risk assessment, and regulatory approval of new substances.	Heavy metal speciation is the analytical and geochemical determination of the chemical forms (species) and partitioning of toxic metals (lead, cadmium, chromium, zinc, copper) in soil, sediment, and water. Metal bioavailability—the fraction accessible to organisms—depends critically on speciation: metal bound to soil organic matter or iron oxides is immobile and non-bioavailable; dissolved or exchangeable metal is highly bioavailable and toxic. Speciation assessment informs remediation design, risk assessment, and contaminant fate prediction.