مقایسهٔ روشها
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| طیفسنجی فروسرخ تبدیل فوریه بازتاب کل تعدیلشده (ATR-FTIR)× | پراکنش دورانی (Circular Dichroism)× | رزونانس پلاسمون سطحی× | |
|---|---|---|---|
| حوزه | طیفسنجی | طیفسنجی | طیفسنجی |
| خانواده | Process / pipeline | Process / pipeline | Process / pipeline |
| سال پیدایش≠ | 1961 | 1969 | 1971 |
| پدیدآور≠ | Joop Fahrenfort | Jean-Claude Fasman | Erich Kretschmann |
| نوع≠ | Vibrational spectroscopy technique | Spectroscopic method | Optical technique |
| منبع بنیادین≠ | Harrick, N. J. (1960). Study of physics of internal reflection from metals. Journal of Physics and Chemistry of Solids, 13(2), 143-155. link ↗ | Greenfield, N. J., & Fasman, G. D. (1969). Computed circular dichroism spectra for protein secondary structures. Biochemistry, 8(10), 4108-4116. DOI ↗ | Kretschmann, E. (1971). Determination of optical constants of metals by excitation of surface plasmons. Zeitschrift für Physik, 241(4), 313-324. link ↗ |
| نامهای دیگر | ATR-IR, attenuated total reflectance, FTIR spectroscopy | CD spectroscopy, circular dichroism, CD analysis | SPR, surface plasmon, SPR biosensing |
| مرتبط | 3 | 3 | 3 |
| خلاصه≠ | Attenuated Total Reflectance (ATR) Fourier Transform Infrared (FTIR) spectroscopy is a variant of conventional FTIR that measures infrared absorption through evanescent-wave interrogation of samples in direct contact with a high-refractive-index crystal. Developed by Harrick and Fahrenfort in the 1960s, ATR-FTIR is now the dominant form of FTIR spectroscopy, enabling rapid, non-destructive characterization of organic compounds, polymers, coatings, and biological materials without extensive sample preparation. | Circular Dichroism (CD) spectroscopy measures the differential absorption of left- and right-circularly polarized light by optically active molecules, particularly proteins and nucleic acids. Introduced by Greenfield and Fasman in 1969, CD is a rapid, non-destructive technique for characterizing secondary structure (alpha-helix, beta-sheet), monitoring protein folding transitions, and assessing conformational changes in response to pH, temperature, or ligand binding. | Surface Plasmon Resonance (SPR) is a real-time, label-free technique for detecting and monitoring biomolecular interactions at a sensor surface by measuring changes in the refractive index caused by ligand binding. Developed by Kretschmann in 1971 and applied to biosensing by Liedberg, Nylander, and Lundström in 1983, SPR is now a gold standard for measuring binding kinetics (association and dissociation rates) and equilibrium binding constants in protein interactions, antibody-antigen recognition, and drug discovery. |
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