Methoden vergleichen
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| Radioimmunassay (RIA)× | Durchflusszytometrie-Analyse× | |
|---|---|---|
| Fachgebiet≠ | Veterinärwissenschaft | Pharmakologie |
| Familie | Process / pipeline | Process / pipeline |
| Entstehungsjahr≠ | 1959–1960 | 1976 |
| Urheber≠ | Rosalyn Yalow and Solomon Berson | Leonard Herzenberg |
| Typ≠ | Quantitative immunological assay | cell analysis and sorting |
| Wegweisende Quelle≠ | Yalow, R. S., & Berson, S. A. (1960). Immunoassay of endogenous plasma insulin in man. Journal of Clinical Investigation, 39(7), 1157–1175. DOI ↗ | Herzenberg, L. A., Parks, D., Sahaf, B., Perez, O., Roederer, M., & Herzenberg, L. A. (2002). The history and future of the fluorescence-activated cell sorter and flow cytometry: a view from Stanford. Clinical Chemistry, 48(10), 1819-1827. DOI ↗ |
| Aliasnamen≠ | RIA, radioisotope immunoassay, isotope immunoassay, radioligand assay | FACS, fluorescence-activated cell sorting, cell analysis |
| Verwandt≠ | 1 | 3 |
| Zusammenfassung≠ | Radioimmunoassay (RIA) is a highly sensitive, quantitative laboratory technique that measures the concentration of a specific antigen — such as a hormone, drug, or pathogen-derived protein — in a biological sample by exploiting competitive binding between a radiolabelled antigen and the sample antigen for a limited supply of specific antibody. Developed in the late 1950s, RIA is widely used in veterinary science, endocrinology, pharmacology, and clinical diagnostics. | Flow cytometry is a laser-based technology for analyzing and sorting individual cells based on fluorescent markers. Developed by Leonard Herzenberg in the 1970s, flow cytometry enables rapid assessment of cell phenotype, drug effects on cell populations, and therapeutic cell characterization in immunology and hematology. |
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