Methoden vergleichen
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| MTT/MTS Assay× | Scratch-Wound-Assay× | |
|---|---|---|
| Fachgebiet | Biomaterialien | Biomaterialien |
| Familie | Process / pipeline | Process / pipeline |
| Entstehungsjahr≠ | 1983 | 2007 |
| Urheber≠ | Tatsuro Mosmann | Liang, Park, and Guan |
| Typ≠ | Colorimetric assay | Migration assessment |
| Wegweisende Quelle≠ | Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63. DOI ↗ | Liang, C. C., Park, A. Y., & Guan, J. L. (2007). In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nature Protocols, 2(2), 329-333. DOI ↗ |
| Aliasnamen | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay | wound healing assay, gap closure assay, migration assay |
| Verwandt | 4 | 4 |
| Zusammenfassung≠ | The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development. | The scratch wound assay (also called the wound healing assay or gap closure assay) is a simple, cost-effective method for measuring cell migration in vitro. Developed and standardized by Liang, Park, and Guan in 2007, the assay involves creating a defined gap (wound) in a monolayer of confluent cells using a pipette tip or specialized tool, then monitoring the rate at which cells migrate into the gap over hours to days. The scratch wound assay is widely used to evaluate the effects of growth factors, inhibitory compounds, and biomaterial extracts on cell motility and wound healing potential. |
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