Methoden vergleichen

	Imaging Mass Cytometry	OCT-Angiographie
Fachgebiet	Medizinische Bildgebung	Medizinische Bildgebung
Familie	Process / pipeline	Process / pipeline
Entstehungsjahr	2014	2012
Urheber	Bernd Bodenmiller	Yali Jia
Typ	Multiplexed single-cell imaging by mass spectrometry	Optical imaging technique for vasculature visualization
Wegweisende Quelle	Giesen, C., Wang, H. A., Schapiro, D., et al. (2014). Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry. Nature Methods, 11(4), 417-422.	Jia, Y., Tan, O., Tokayer, J., et al. (2012). Split-spectrum amplitude-decorrelation angiography with optical coherence tomography. Optics Express, 20(4), 4710-4725.
Aliasnamen	IMC, mass cytometry, multiplex ion beam imaging, MIBI	OCTA, OCT-A
Verwandt	5	5
Zusammenfassung	Imaging Mass Cytometry (IMC) is a multiplexed proteomics technique that maps the subcellular localization of up to 40-50 proteins in tissue sections simultaneously using mass spectrometry detection. Developed by Bodenmiller and colleagues in 2014, IMC combines the single-cell imaging power of immunofluorescence with the multiplexing capacity of mass cytometry, enabling comprehensive analysis of cell types, states, and spatial interactions within tissue microenvironments. IMC has emerged as a powerful tool in immuno-oncology, immunobiology, and tissue biology for dissecting cellular heterogeneity and spatial organization.	Optical Coherence Tomography Angiography (OCTA) is a non-invasive imaging technique that visualizes the microvasculature in the retina and choroid by detecting motion contrast from flowing blood. Developed by Jia and colleagues in 2012, OCTA uses repeated OCT scans of the same tissue location to identify blood flow based on the decorrelation signal. It has become a critical diagnostic tool in ophthalmology for detecting retinal and macular diseases without requiring fluorescein injection.