Methoden vergleichen

	GPC/SEC	Schwellung und Degradation
Fachgebiet	Biomaterialien	Biomaterialien
Familie	Process / pipeline	Process / pipeline
Entstehungsjahr	1962	1960
Urheber	Moore and Debye	Wichterle and Lim
Typ	Chromatographic analysis	Kinetic assay
Wegweisende Quelle	Striegel, A. M., Yau, W. W., Kirkland, J. J., & Bly, D. D. (2009). Modern size-exclusion liquid chromatography: practice and theory. John Wiley & Sons.	Wichterle, O., & Lim, D. (1960). Hydrophilic gels for biological use. Nature, 185(4706), 117-118.
Aliasnamen	size exclusion chromatography, molecular weight determination, polymer characterization	hydrogel swelling, polymer degradation, mass loss assay
Verwandt	3	4
Zusammenfassung	Gel permeation chromatography (GPC), also known as size exclusion chromatography (SEC), is an analytical technique for determining the molecular weight distribution (MWD) and average molecular weight (Mw, Mn) of polymers. The method separates polymer molecules by their hydrodynamic size as they pass through a porous chromatography column: larger molecules elute first (excluded from pores), while smaller molecules are retained longer. Developed by Moore and colleagues in the 1960s, GPC/SEC is now the standard method for characterizing polymer chains, assessing polymer degradation over time, and verifying batch consistency in biomaterial production.	The swelling and degradation assay measures how biomaterial scaffolds absorb water (swelling) and lose mass over time due to degradation. Developed by Wichterle and Lim in 1960 for hydrogels, the assay is fundamental for characterizing hydrogels, synthetic polymers, and composite scaffolds intended for tissue engineering. The assay provides quantitative data on swelling kinetics (equilibrium water content, swelling ratio), degradation kinetics (mass loss rate, half-life), and mechanisms of degradation (chain scission, enzymatic breakdown).