Minimum Inhibitory Concentration Assay
The Minimum Inhibitory Concentration (MIC) assay is a quantitative in vitro method that determines the lowest concentration of an antimicrobial agent — such as a food preservative, essential oil, or synthetic antibiotic — that visibly inhibits the growth of a target microorganism. Widely used in food science, microbiology, and pharmaceutical research, it provides a reproducible numerical threshold that guides formulation, safety assessment, and regulatory compliance decisions.
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- Clinical and Laboratory Standards Institute (CLSI). (2018). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically, 11th ed. CLSI standard M07. Wayne, PA: CLSI. · URL
- Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71-79. · DOI 10.1016/j.jpha.2015.11.005
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