Sammenlign metoder

	CAM-analyse	Elektrospinning	Live/Dead-assay	MTT/MTS-assayen
Fagområde	Biomaterialer	Biomaterialer	Biomaterialer	Biomaterialer
Familie	Process / pipeline	Process / pipeline	Process / pipeline	Process / pipeline
Oprindelsesår	1974	1934	2000	1983
Ophavsperson	Judah Folkman	Anton Formhals	Invitrogen/Molecular Probes	Tatsuro Mosmann
Type	Developmental biology assay	Fiber fabrication process	Dual-dye viability assay	Colorimetric assay
Oprindelig kilde	Folkman, J. (1974). Tumor angiogenesis: therapeutic implications. New England Journal of Medicine, 285(21), 1182-1186.	Formhals, A. (1934). Process and apparatus for preparing artificial threads. U.S. Patent 1,975,504.	Molecular Probes (2004). LIVE/DEAD Viability/Cytotoxicity Kit user guide. Invitrogen Corporation.	Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2), 55-63.
Aliasser	chick embryo chorioallantoic membrane, angiogenesis assay, CAM angiogenesis model	electrospun fiber production, electrostatic fiber spinning	calcein-AM/propidium iodide, SYTO/PI staining, fluorescent viability stain	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, tetrazolium assay, mitochondrial activity assay
Relaterede	4	3	4	4
Resumé	The chorioallantoic membrane (CAM) assay is a well-established in vivo model for studying angiogenesis (new blood vessel formation) and evaluating the pro- or anti-angiogenic properties of biomaterials, drugs, and bioactive molecules. Developed by Judah Folkman in the 1970s, the assay uses the highly vascularized CAM of developing chick embryos as a platform for implanting test materials and observing vascular response. The CAM provides a transparent, immunologically naive microenvironment with rapid and reproducible neovascularization, making it ideal for screening angiogenic potential and assessing biomaterial biocompatibility.	Electrospinning is an electrostatic fiber fabrication process that uses a high electric field to draw polymer solutions or melts into nanoscale fibers. Developed by Anton Formhals in the 1930s and refined by researchers including Darrell Reneker in the 1990s, the technique has become foundational to biomaterials engineering, enabling the creation of porous scaffolds for tissue engineering and drug delivery systems.	The Live/Dead assay is a fluorescence-based method for simultaneously identifying live and dead cells using two complementary dyes. The assay combines calcein-AM (or SYTO fluorophores), which generates bright green fluorescence in living cells with intact esterase activity, with propidium iodide (PI), which produces red fluorescence in dead cells with compromised membrane integrity. Commercially developed by Molecular Probes and now part of Thermo Fisher's portfolio, the Live/Dead kit is widely used to evaluate cell viability on biomaterial scaffolds, in tissue constructs, and following drug or toxin exposure.	The MTT assay, introduced by Tatsuro Mosmann in 1983, is a colorimetric method for quantifying cell viability and proliferation by measuring mitochondrial metabolic activity. The method detects the conversion of the water-soluble tetrazolium salt MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) by active mitochondria, producing an insoluble purple formazan precipitate proportional to the number of viable cells. The related MTS assay, which does not require solubilization, offers improved kinetics and is now widely adopted in both academic research and pharmaceutical development.